apc a750 anti mouse cd45 antibody Search Results


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HMGB1 CKO improves Th1/Th2 inflammation in AT-II cells of asthmatic mice. Isolated AT-II cells from lung single cell suspensions were labeled, and then using flow cytometry analyzed them. A , B Schematic diagram of the gating strategy of AT-II cells <t>(CD45.2</t> − /CD31 − /EpCAM + /proSP-C + /MHCII + ), and further analysis of HMGB1, TLR4, DRP1, IL-1β, IL-4, IFN-γ, and TIM3, and cell proportion. * p < 0.05, ** p < 0.01, *** p < 0.001
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HMGB1 CKO improves Th1/Th2 inflammation in AT-II cells of asthmatic mice. Isolated AT-II cells from lung single cell suspensions were labeled, and then using flow cytometry analyzed them. A , B Schematic diagram of the gating strategy of AT-II cells <t>(CD45.2</t> − /CD31 − /EpCAM + /proSP-C + /MHCII + ), and further analysis of HMGB1, TLR4, DRP1, IL-1β, IL-4, IFN-γ, and TIM3, and cell proportion. * p < 0.05, ** p < 0.01, *** p < 0.001
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Bioss endomucin polyclonal antibody, alexa fluor 750 conjugated
HMGB1 CKO improves Th1/Th2 inflammation in AT-II cells of asthmatic mice. Isolated AT-II cells from lung single cell suspensions were labeled, and then using flow cytometry analyzed them. A , B Schematic diagram of the gating strategy of AT-II cells <t>(CD45.2</t> − /CD31 − /EpCAM + /proSP-C + /MHCII + ), and further analysis of HMGB1, TLR4, DRP1, IL-1β, IL-4, IFN-γ, and TIM3, and cell proportion. * p < 0.05, ** p < 0.01, *** p < 0.001
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Becton Dickinson apc-conjugated anti-cd38
HMGB1 CKO improves Th1/Th2 inflammation in AT-II cells of asthmatic mice. Isolated AT-II cells from lung single cell suspensions were labeled, and then using flow cytometry analyzed them. A , B Schematic diagram of the gating strategy of AT-II cells <t>(CD45.2</t> − /CD31 − /EpCAM + /proSP-C + /MHCII + ), and further analysis of HMGB1, TLR4, DRP1, IL-1β, IL-4, IFN-γ, and TIM3, and cell proportion. * p < 0.05, ** p < 0.01, *** p < 0.001
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HMGB1 CKO improves Th1/Th2 inflammation in AT-II cells of asthmatic mice. Isolated AT-II cells from lung single cell suspensions were labeled, and then using flow cytometry analyzed them. A , B Schematic diagram of the gating strategy of AT-II cells <t>(CD45.2</t> − /CD31 − /EpCAM + /proSP-C + /MHCII + ), and further analysis of HMGB1, TLR4, DRP1, IL-1β, IL-4, IFN-γ, and TIM3, and cell proportion. * p < 0.05, ** p < 0.01, *** p < 0.001
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Becton Dickinson anti-b220(ra3-6b2)-pacblue
HMGB1 CKO improves Th1/Th2 inflammation in AT-II cells of asthmatic mice. Isolated AT-II cells from lung single cell suspensions were labeled, and then using flow cytometry analyzed them. A , B Schematic diagram of the gating strategy of AT-II cells <t>(CD45.2</t> − /CD31 − /EpCAM + /proSP-C + /MHCII + ), and further analysis of HMGB1, TLR4, DRP1, IL-1β, IL-4, IFN-γ, and TIM3, and cell proportion. * p < 0.05, ** p < 0.01, *** p < 0.001
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Bioss leptin receptor polyclonal antibody
HMGB1 CKO improves Th1/Th2 inflammation in AT-II cells of asthmatic mice. Isolated AT-II cells from lung single cell suspensions were labeled, and then using flow cytometry analyzed them. A , B Schematic diagram of the gating strategy of AT-II cells <t>(CD45.2</t> − /CD31 − /EpCAM + /proSP-C + /MHCII + ), and further analysis of HMGB1, TLR4, DRP1, IL-1β, IL-4, IFN-γ, and TIM3, and cell proportion. * p < 0.05, ** p < 0.01, *** p < 0.001
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Bioss 8-ohdg polyclonal antibody
HMGB1 CKO improves Th1/Th2 inflammation in AT-II cells of asthmatic mice. Isolated AT-II cells from lung single cell suspensions were labeled, and then using flow cytometry analyzed them. A , B Schematic diagram of the gating strategy of AT-II cells <t>(CD45.2</t> − /CD31 − /EpCAM + /proSP-C + /MHCII + ), and further analysis of HMGB1, TLR4, DRP1, IL-1β, IL-4, IFN-γ, and TIM3, and cell proportion. * p < 0.05, ** p < 0.01, *** p < 0.001
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Bioss pan cytokeratin polyclonal antibody
HMGB1 CKO improves Th1/Th2 inflammation in AT-II cells of asthmatic mice. Isolated AT-II cells from lung single cell suspensions were labeled, and then using flow cytometry analyzed them. A , B Schematic diagram of the gating strategy of AT-II cells <t>(CD45.2</t> − /CD31 − /EpCAM + /proSP-C + /MHCII + ), and further analysis of HMGB1, TLR4, DRP1, IL-1β, IL-4, IFN-γ, and TIM3, and cell proportion. * p < 0.05, ** p < 0.01, *** p < 0.001
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Bioss prrsv m protein polyclonal antibody
HMGB1 CKO improves Th1/Th2 inflammation in AT-II cells of asthmatic mice. Isolated AT-II cells from lung single cell suspensions were labeled, and then using flow cytometry analyzed them. A , B Schematic diagram of the gating strategy of AT-II cells <t>(CD45.2</t> − /CD31 − /EpCAM + /proSP-C + /MHCII + ), and further analysis of HMGB1, TLR4, DRP1, IL-1β, IL-4, IFN-γ, and TIM3, and cell proportion. * p < 0.05, ** p < 0.01, *** p < 0.001
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HMGB1 CKO improves Th1/Th2 inflammation in AT-II cells of asthmatic mice. Isolated AT-II cells from lung single cell suspensions were labeled, and then using flow cytometry analyzed them. A , B Schematic diagram of the gating strategy of AT-II cells (CD45.2 − /CD31 − /EpCAM + /proSP-C + /MHCII + ), and further analysis of HMGB1, TLR4, DRP1, IL-1β, IL-4, IFN-γ, and TIM3, and cell proportion. * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Mechanism of miR-130b-3p in relieving airway inflammation in asthma through HMGB1-TLR4-DRP1 axis

doi: 10.1007/s00018-024-05529-0

Figure Lengend Snippet: HMGB1 CKO improves Th1/Th2 inflammation in AT-II cells of asthmatic mice. Isolated AT-II cells from lung single cell suspensions were labeled, and then using flow cytometry analyzed them. A , B Schematic diagram of the gating strategy of AT-II cells (CD45.2 − /CD31 − /EpCAM + /proSP-C + /MHCII + ), and further analysis of HMGB1, TLR4, DRP1, IL-1β, IL-4, IFN-γ, and TIM3, and cell proportion. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: To label ATII cells, we incubated single cell suspensions with surface antibodies including APC-A750-CD45.2 (leukocytes, 25 −0454-U025, MBL), PC7-CD31 (endothelial cell, 561410, BD), Epcam (epithelial cell, 563477, BD, USA), PC5.5-MHC-II (ATII cell surface marker, 50–5321-U025, MBL), after processed with perforation and membrane rupture buffer, cells were incubated with FITC-proSP-C (surfactant protein C, ATII cell-specific surfactant protein, ab90713, abcam) [ ].

Techniques: Isolation, Labeling, Flow Cytometry